LOCUS       40924_49137        4141 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD025, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4141)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4141)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..877
                     /note="S7942NS3-RA; right arm of the homologous
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     rep_origin      914..1502
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            complement(1743..1852)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_recomb     2039..2930
                     /note="S7942NS3-LA; left arm of the homologous
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     misc_feature    2931..2936
                     /label=NheI
                     /note="NheI"
     misc_feature    2937..2957
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2955..2960
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(3084..3941)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3942..4046)
                     /label=AmpR promoter
     misc_feature    4072..4077
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagcgacgt cgggggcccc
       61 cggggggatt ctagacgtga gccgtagtcc cgtcgccaga cgcgggtgcc cacgggcgtc
      121 gtcaggatgt ccgtaattga ctgccggagg tggtcaatgc ccttcagctc cttgccactg
      181 tcacggctca tgcctcgggt cattagtcgc ccgctccggt atcttcactg gcttcgatga
      241 ttgccgcccc gcagctgcag aggtcaccga tccgagcagt cggcctctgg ttggtaaaga
      301 ccgtgcgact gccggtgatg atcgtgttca agccatgcag ggggcaggcg tgaaggtcat
      361 cctttcgggc cacggggcgg ctgttgacga aggtgtcgtc gctcccggtg atgatgatcc
      421 cgccgtgatc ggtcacgtcg tttagtcgag cgatgcctgg cgtcgtagtc acgggtttag
      481 gtcaatacga cttgcggtca ctgtaacgtt gccctcggcg gtcacgttaa cgtcgccttg
      541 ggcttcgact tgcgcctcct gcacaaggat cacaatccgt ccttgggctg cggtgaggtc
      601 gatcttgtac tcatgcgctt cgcggtcgta ctggatgatt gagtcatcct cgaactgcgt
      661 cttttggatc gtttctttgt cctcgatctg ggggtagtca gtcgagaacg cgccgggcat
      721 cgcgaagccc tgactgatct cgccggaggg ggccatcacg acgacggcct caccgacctc
      781 gggcgcccac cagaaccgat ccttgcccgc tcgctgcgtg agccacggaa tccagtcagt
      841 gaggagcagc gcctcgccgc tctcctcgtc ttcctcgagc gtcagacccc gtagaaaaga
      901 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      961 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1021 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     1081 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1141 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1201 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1261 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1321 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1381 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1441 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1501 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1561 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1621 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1681 aagactggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt
     1741 agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac
     1801 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg
     1861 ccgttggata caccaaggaa agtctacgcg aaccctttgg caaaatcctg tatatcgtgc
     1921 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc
     1981 ggaagatccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctccgcggcg
     2041 tcgtgatgtc gaaacccaag ccaccctcag aggtgaaggc cgcttcgagc acatcgggaa
     2101 gcgtgtcgac gacggtgctc ggtgcctcgg gtaagagcca gatagatgcg gtgacgttca
     2161 ccgtcgcgat cgtggcggga accacggtca ctgcgtccgt tacaactcgc acgtcgtcgg
     2221 ccagcacgac tgactcgacg gcttcgatta gcccgggaga ggcaaggcca tcaggctcgg
     2281 tagacagaat gctgattaac acctcgccgg gagcagggct gctcacagcc gcgtccttca
     2341 cccgtgggtc agccgtcagc gcttgatagc ggtaccaggc cgcaccgcct gcggtcgagc
     2401 tgcccttgat ccgctcgatg gtgcgatcgc gaagctcgcc atccggctca ttcggctgcc
     2461 gcaccacgcc gtagaacgcg gcaaggttgt cgaggtcggg gccgtttgag tagcgaagca
     2521 gtgtcgcaag aagtgcgtcg ttgatccgct gacgcaggat cagctcgcgg gctgcgcaga
     2581 cctccagcag cttgatgacc gggtcggatt cgaggatggc ggtgtagctg gcgtcacgcg
     2641 atcgcaggtc gtcgatcaag tcctgcagga tcagttcaaa gtccagcgct tcgatgatgg
     2701 tgggcgcggg aatcgtagca aagtcaagaa cggtcatgag acgactaagc cctccagcgt
     2761 gatacgcctg ccctcgggga tgtagtagcc gatcaggttc agctcgactt gaccagctgc
     2821 gctggctgag acgatgcgaa ccttctccag cttcagccgt ggctcccagc gatcgagcgc
     2881 ttcagctgtg gccgccacca ggtcaacgat gagggactgg ttgatcggtc gctagcatcg
     2941 cgcgcgcgcg cgcggacgtc ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3001 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3061 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3121 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3181 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3241 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3301 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3361 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3421 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3481 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3541 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3601 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3661 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3721 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3781 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3841 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3901 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3961 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4021 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     4081 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     4141 c
//
